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umr106 rat osteogenic sarcoma cells  (ATCC)


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    ATCC umr106 rat osteogenic sarcoma cells
    Parathyroid hormone (PTH) regulates fibroblast growth factor 23 ( Fgf23 ) and Sost expression in <t>UMR106</t> cells. A and B, Time-dependent changes in A, Fgf23 , and B, Sost expression levels in UMR106 cells after treatment with 10 –7 M 1-34 PTH at 4, 24, 48, 96, and 168 hours. C and D, Concentration-dependent changes in C, Fgf23 , and D, Sost expression levels in UMR106 cells treated with 1-34 PTH for 24 hours. Results are relative to 18S messenger RNA and are presented as the fold-change in expression compared with control (vehicle-treated) cells. Data are shown as the mean ± SD (n = 3); * P less than .05, by t test or analysis of variance with Dunnett test. E, Western blot analysis of FGF23 and sclerostin in UMR106 cells after treatment with 10 –7 M 1-34 PTH for 24 hours.
    Umr106 Rat Osteogenic Sarcoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 411 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/umr106 rat osteogenic sarcoma cells/product/ATCC
    Average 95 stars, based on 411 article reviews
    umr106 rat osteogenic sarcoma cells - by Bioz Stars, 2026-05
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    1) Product Images from "Parathyroid Hormone Regulates Circulating Levels of Sclerostin and FGF23 in a Primary Hyperparathyroidism Model"

    Article Title: Parathyroid Hormone Regulates Circulating Levels of Sclerostin and FGF23 in a Primary Hyperparathyroidism Model

    Journal: Journal of the Endocrine Society

    doi: 10.1210/jendso/bvac027

    Parathyroid hormone (PTH) regulates fibroblast growth factor 23 ( Fgf23 ) and Sost expression in UMR106 cells. A and B, Time-dependent changes in A, Fgf23 , and B, Sost expression levels in UMR106 cells after treatment with 10 –7 M 1-34 PTH at 4, 24, 48, 96, and 168 hours. C and D, Concentration-dependent changes in C, Fgf23 , and D, Sost expression levels in UMR106 cells treated with 1-34 PTH for 24 hours. Results are relative to 18S messenger RNA and are presented as the fold-change in expression compared with control (vehicle-treated) cells. Data are shown as the mean ± SD (n = 3); * P less than .05, by t test or analysis of variance with Dunnett test. E, Western blot analysis of FGF23 and sclerostin in UMR106 cells after treatment with 10 –7 M 1-34 PTH for 24 hours.
    Figure Legend Snippet: Parathyroid hormone (PTH) regulates fibroblast growth factor 23 ( Fgf23 ) and Sost expression in UMR106 cells. A and B, Time-dependent changes in A, Fgf23 , and B, Sost expression levels in UMR106 cells after treatment with 10 –7 M 1-34 PTH at 4, 24, 48, 96, and 168 hours. C and D, Concentration-dependent changes in C, Fgf23 , and D, Sost expression levels in UMR106 cells treated with 1-34 PTH for 24 hours. Results are relative to 18S messenger RNA and are presented as the fold-change in expression compared with control (vehicle-treated) cells. Data are shown as the mean ± SD (n = 3); * P less than .05, by t test or analysis of variance with Dunnett test. E, Western blot analysis of FGF23 and sclerostin in UMR106 cells after treatment with 10 –7 M 1-34 PTH for 24 hours.

    Techniques Used: Expressing, Concentration Assay, Control, Western Blot

    The regulation of fibroblast growth factor 23 ( Fgf23 ) by the parathyroid hormone/protein kinase A (PTH/PKA) pathway and sclerostin in UMR106 cells. A, Fgf23 , and B, Sost expression in UMR106 cells after treatment with 10 –6 M to 10 –4 M forskolin for 24 hours. C, Fgf23 , and D, Sost expression in UMR106 cells after treatment with 10 –7 M 1-34 PTH with or without 10 µM H89 for 24 hours. E, FGF23 gene, and F, FGF23 protein expression in UMR106 cells after treatment with 10 –7 M 1-34 PTH with or without 4.4 × 10 –8 M sclerostin for 24 hours. Results are relative to 18S messenger RNA and are presented as the fold-change in expression compared with control (vehicle-treated) cells. Data are presented as the mean ± SD (n = 3); * P less than .05, by analysis of variance with Tukey-Kramer test.
    Figure Legend Snippet: The regulation of fibroblast growth factor 23 ( Fgf23 ) by the parathyroid hormone/protein kinase A (PTH/PKA) pathway and sclerostin in UMR106 cells. A, Fgf23 , and B, Sost expression in UMR106 cells after treatment with 10 –6 M to 10 –4 M forskolin for 24 hours. C, Fgf23 , and D, Sost expression in UMR106 cells after treatment with 10 –7 M 1-34 PTH with or without 10 µM H89 for 24 hours. E, FGF23 gene, and F, FGF23 protein expression in UMR106 cells after treatment with 10 –7 M 1-34 PTH with or without 4.4 × 10 –8 M sclerostin for 24 hours. Results are relative to 18S messenger RNA and are presented as the fold-change in expression compared with control (vehicle-treated) cells. Data are presented as the mean ± SD (n = 3); * P less than .05, by analysis of variance with Tukey-Kramer test.

    Techniques Used: Expressing, Control



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    Parathyroid hormone (PTH) regulates fibroblast growth factor 23 ( Fgf23 ) and Sost expression in <t>UMR106</t> cells. A and B, Time-dependent changes in A, Fgf23 , and B, Sost expression levels in UMR106 cells after treatment with 10 –7 M 1-34 PTH at 4, 24, 48, 96, and 168 hours. C and D, Concentration-dependent changes in C, Fgf23 , and D, Sost expression levels in UMR106 cells treated with 1-34 PTH for 24 hours. Results are relative to 18S messenger RNA and are presented as the fold-change in expression compared with control (vehicle-treated) cells. Data are shown as the mean ± SD (n = 3); * P less than .05, by t test or analysis of variance with Dunnett test. E, Western blot analysis of FGF23 and sclerostin in UMR106 cells after treatment with 10 –7 M 1-34 PTH for 24 hours.
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    Image Search Results


    Parathyroid hormone (PTH) regulates fibroblast growth factor 23 ( Fgf23 ) and Sost expression in UMR106 cells. A and B, Time-dependent changes in A, Fgf23 , and B, Sost expression levels in UMR106 cells after treatment with 10 –7 M 1-34 PTH at 4, 24, 48, 96, and 168 hours. C and D, Concentration-dependent changes in C, Fgf23 , and D, Sost expression levels in UMR106 cells treated with 1-34 PTH for 24 hours. Results are relative to 18S messenger RNA and are presented as the fold-change in expression compared with control (vehicle-treated) cells. Data are shown as the mean ± SD (n = 3); * P less than .05, by t test or analysis of variance with Dunnett test. E, Western blot analysis of FGF23 and sclerostin in UMR106 cells after treatment with 10 –7 M 1-34 PTH for 24 hours.

    Journal: Journal of the Endocrine Society

    Article Title: Parathyroid Hormone Regulates Circulating Levels of Sclerostin and FGF23 in a Primary Hyperparathyroidism Model

    doi: 10.1210/jendso/bvac027

    Figure Lengend Snippet: Parathyroid hormone (PTH) regulates fibroblast growth factor 23 ( Fgf23 ) and Sost expression in UMR106 cells. A and B, Time-dependent changes in A, Fgf23 , and B, Sost expression levels in UMR106 cells after treatment with 10 –7 M 1-34 PTH at 4, 24, 48, 96, and 168 hours. C and D, Concentration-dependent changes in C, Fgf23 , and D, Sost expression levels in UMR106 cells treated with 1-34 PTH for 24 hours. Results are relative to 18S messenger RNA and are presented as the fold-change in expression compared with control (vehicle-treated) cells. Data are shown as the mean ± SD (n = 3); * P less than .05, by t test or analysis of variance with Dunnett test. E, Western blot analysis of FGF23 and sclerostin in UMR106 cells after treatment with 10 –7 M 1-34 PTH for 24 hours.

    Article Snippet: UMR106 rat osteogenic sarcoma cells (CRL-1661; American Type Culture Collection) [ ] were cultured in α-Minimum Essential Medium (α-MEM; Gibco) containing 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco) at 37 °C in a humidified atmosphere of 5% CO 2 .

    Techniques: Expressing, Concentration Assay, Control, Western Blot

    The regulation of fibroblast growth factor 23 ( Fgf23 ) by the parathyroid hormone/protein kinase A (PTH/PKA) pathway and sclerostin in UMR106 cells. A, Fgf23 , and B, Sost expression in UMR106 cells after treatment with 10 –6 M to 10 –4 M forskolin for 24 hours. C, Fgf23 , and D, Sost expression in UMR106 cells after treatment with 10 –7 M 1-34 PTH with or without 10 µM H89 for 24 hours. E, FGF23 gene, and F, FGF23 protein expression in UMR106 cells after treatment with 10 –7 M 1-34 PTH with or without 4.4 × 10 –8 M sclerostin for 24 hours. Results are relative to 18S messenger RNA and are presented as the fold-change in expression compared with control (vehicle-treated) cells. Data are presented as the mean ± SD (n = 3); * P less than .05, by analysis of variance with Tukey-Kramer test.

    Journal: Journal of the Endocrine Society

    Article Title: Parathyroid Hormone Regulates Circulating Levels of Sclerostin and FGF23 in a Primary Hyperparathyroidism Model

    doi: 10.1210/jendso/bvac027

    Figure Lengend Snippet: The regulation of fibroblast growth factor 23 ( Fgf23 ) by the parathyroid hormone/protein kinase A (PTH/PKA) pathway and sclerostin in UMR106 cells. A, Fgf23 , and B, Sost expression in UMR106 cells after treatment with 10 –6 M to 10 –4 M forskolin for 24 hours. C, Fgf23 , and D, Sost expression in UMR106 cells after treatment with 10 –7 M 1-34 PTH with or without 10 µM H89 for 24 hours. E, FGF23 gene, and F, FGF23 protein expression in UMR106 cells after treatment with 10 –7 M 1-34 PTH with or without 4.4 × 10 –8 M sclerostin for 24 hours. Results are relative to 18S messenger RNA and are presented as the fold-change in expression compared with control (vehicle-treated) cells. Data are presented as the mean ± SD (n = 3); * P less than .05, by analysis of variance with Tukey-Kramer test.

    Article Snippet: UMR106 rat osteogenic sarcoma cells (CRL-1661; American Type Culture Collection) [ ] were cultured in α-Minimum Essential Medium (α-MEM; Gibco) containing 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco) at 37 °C in a humidified atmosphere of 5% CO 2 .

    Techniques: Expressing, Control